Visfatin expression and genetic polymorphism in patients with traumatic brain injury.

OBJECTIVE To discover the expression of visfatin and its genetic mutation and clinical significance in patients with traumatic brain injury. METHODS 99 cases of traumatic brain injury patients admitted to Chongqing Emergency Medical Center from June 2013 to December 2014. There were 56 male and 43 female with an average age of 45.2 years. Through diagnosis, the 99 patient's conditions were all relatively in accordance with traumatic brain injury standard; meanwhile, the patients were divided into mild, moderate and severe traumatic brain injury according to GCS criteria when they admitted to clinics. 33 people were enrolled in each group. To highlight the results, 40 healthy people were enrolled in the control group with 25 males and 15 females. ELISA was used to detect serum visfatin level in 99 patients. Single base extension was used to detect visfatin gene promoter -1535C/T polymorphism. RESULTS By examination we found that visfatin gene -1535C > T locus had three genotypes: TT, TC and CC, respectively. By examination we obtained that genotype distribution and allele frequency showed no significance between the experimental group and the control group (P > 0.05). Through analysis we can get that serum visfatin level in patients with traumatic brain injury was significantly higher than that in the control group. CC genotype are mostly patients with severe traumatic brain injury, and its serum visfatin level was higher than that with CT and TT genotype (all P < 0.05). CT and TT genotype carriers were most mild and moderate traumatic brain injury patients. CONCLUSION Expression of visfatin and its gene mutation in patients with traumatic brain injury were closely related to the severity of the disease.